gutB [2018-05-23 18:37:05]

D-sorbitol dehydrogenase

locus: BSU06150

pI: 5.21

mw: 38.23 kDa

protein length: 353 aa

gene length: 1059 bp

function: glucitol utilization

product: D-sorbitol dehydrogenase

essential: no

ec: 1.1.1.14

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.11|Utilization of glucitol]

Gene

Coordinates: 667,466 → 668,527

The protein

Catalyzed reaction/ biological activity

• L-iditol + NAD⁺ = L-sorbose + NADH (according to Swiss-Prot)

Protein family

• zinc-containing alcohol dehydrogenase family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|2E9D10B0FA5685800007222A5AAD71E975675DB1|gutB]-[gene|B13F7C35FBD55C674E5B19836D32279E7C1A5520|gutP]-[gene|0C4F0C9EF18FB4E9BB5BC3F53F7DDF5F32B51FA5|fruC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8195086], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|926BCA197F259558F72FDCC73998497B9B167D22|GutR]: activation, [Pubmed|8195086], in [regulon|926BCA197F259558F72FDCC73998497B9B167D22|GutR regulon]

  regulation

  • induced by glucitol ([protein|926BCA197F259558F72FDCC73998497B9B167D22|GutR]) [Pubmed|8195086]

Biological materials

Mutant

• BKE06150 (Δ[gene|2E9D10B0FA5685800007222A5AAD71E975675DB1|gutB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGCAAGTTCCTTTCT, downstream forward: _UP4_TGAGTGAACAGGGAGGATCT
• BKK06150 (Δ[gene|2E9D10B0FA5685800007222A5AAD71E975675DB1|gutB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGCAAGTTCCTTTCT, downstream forward: _UP4_TGAGTGAACAGGGAGGATCT

References

• 1460002,12897001,8195086